Community Resources

Microfluidic Devices

The Shredder
Figure 2 of Kasimatis et al. (2018) DOI: 10.1186/s12864-018-4980-7
The Pheromone Arena
Figure 1 of Borne et al. (2017) DOI: 10.1371/journal.pone.0189679
The EjacuChip

The Shredder is a microfluidic dissection device that uses a fine glass needle to slice through the cuticle and testis of males to release stored spermatids.

Please cite DOI: 10.1186/s12864-018-4980-7 to make or modify The Shredder using the EPS file.

The Pheromone Arena is designed to quantify pheromone communication and choice using real time production of pheromones in a controlled environment.

Please cite DOI:10.1371/journal.pone.0189679 to make or modify The Pheromone Arena using the EPS file.

The EjacuChip sequentially loads up to 80 males into individual channels. The chip was designed to induce ejaculations for sperm collection, however, it is also amenable for single worm imaging.

Please acknowledge Katja Kasimatis when accessing The EjacuChip EPS file.

Worm Strains

The Kasimatis lab designation is KRK and allele designation is uva.

NSPF gene family: Deletion and transgene

The NSPF deletion allele (fxDf1) removed nspf-1, nspf-2, and nspf-3 using CRISPR.
To created a tagged trans gene, the promoter sequence for nspf-1 along with a 6xHis-tagged nspf-1 were inserted into the nspf deletion allele background (PX814) using CRISPR.
PX623 fxDf1 II; him-5(e1490) V
VX194 fxDf1 II; fog-2(q71) V
VX300 yfSi1 II (nspf-1p::nspf-1::6xHis::tbb-2 3′ UTR + loxP, II:8420157)

Inducible male sterility

Auxin-inducible spermatogenesis arrest, resulting in hermaphrodite and/or male self-sterility. Degron tag was inserted into the endogenous spe-44 locus using CRISPR.
PX627 fxIs1 I; spe-44(fx110[spe-44::degron]) IV
PX629 fxIs1 I; spe-44(fx110[spe-44::degron]) IV; him-5(e1490) V

Experimental Evolution of Male Reproductive Success

These strains were created in Kasimatis et al. 2022 for isolating evolution of sperm competitive ability. Other strains created in this study are available from the Phillips lab upon request.
PX624 fxSi1 I; spe-44(fx123[spe-44::degron]) IV; fog-2(fx111) V – focal male genotype (pre-mutagenesis)
PX631 fxSi3 I; fxSi4 II; fog-2(q71) V – novel competitor male genotype for bioassays
PX658 fxSi1 I; fxSi4 II; fxSi6 III; spe-44(fx123[spe-44::degron]) IV; fog-2(fx111) V – competitor male genotype

Code

Please see Katja’s GitHub page for project code.